This course is 50% theory and 50% practical in a lab environment.
After an introduction to liquid chromatography, this course addresses solvents & buffers (the mobile phase), pumps, isocratic and gradient elution, sample introduction, analytical columns, HPLC detectors, mass spectrometry including tuning, ionisation, mass analysers, ion detector and data analysis. The knowledge is then used to create methods, perform injections and change parameters to see the effects. The course discusses the need for maintenance along with practicals to carry out maintenance on both an HPLC and an LC-MS system such as the autosampler, injector, columns, detectors, MS tuning, vacuum system, ion source and the mass analyser. A day is spent on troubleshooting an HPLC and LC-MS instrument and learning what problems can occur, with real problem solving on the instruments and data analysis software.
This course combines the five individual hands-on days covering HPLC Theory and Methods, HPLC Maintenance, LC-MS Theory and Methods, LC-MS Maintenance and HPLC & LC-MS Troubleshooting.
After an introduction to liquid chromatography, this course addresses solvents & buffers (the mobile phase), pumps, isocratic and gradient elution, sample introduction, analytical columns, HPLC detectors, mass spectrometry including tuning, ionisation, mass analysers, ion detector and data analysis. The knowledge is then used to create methods, perform injections and change parameters to see the effects. The course discusses the need for maintenance along with practicals to carry out maintenance on both an HPLC and an LC-MS system such as the autosampler, injector, columns, detectors, MS tuning, vacuum system, ion source and the mass analyser. A day is spent on troubleshooting an HPLC and LC-MS instrument and learning what problems can occur, with real problem solving on the instruments and data analysis software.
This course combines the five individual hands-on days covering HPLC Theory and Methods, HPLC Maintenance, LC-MS Theory and Methods, LC-MS Maintenance and HPLC & LC-MS Troubleshooting.
