The Analysis of Polar Molecules
This course is suitable for analysts looking to explore different chromatographic techniques/modes
applicable for the analysis of polar molecules. It will introduce you to a number of possible
techniques that can be employed to retain polar molecules, generally hard to retain using C18
columns in reversed-phase solvent systems. It includes details of new column chemistries suitable
for reversed-phase retention of polars, such as polar embedded columns, as well as alternative
techniques including: ion pairing, mixed-mode, Hydrophilic Interaction Liquid Chromatography
(HILIC), Aqueous Normal Phase (ANP) and Supercritical Fluid Chromatography (SFC). This course
allows you to explore the various options available to expand the polarity range of molecules that
can be retained and discusses how these complement standard reversed-phase methodology.
鈥� Limitations of reversed-phase HPLC 鈥� HILIC
鈥� Alternatives to C18 鈥� Aqueous normal-phase
鈥� Ion pairing 鈥� SFC
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
This course is suitable for analysts looking to explore different chromatographic techniques/modes
applicable for the analysis of polar molecules. It will introduce you to a number of possible
techniques that can be employed to retain polar molecules, generally hard to retain using C18
columns in reversed-phase solvent systems. It includes details of new column chemistries suitable
for reversed-phase retention of polars, such as polar embedded columns, as well as alternative
techniques including: ion pairing, mixed-mode, Hydrophilic Interaction Liquid Chromatography
(HILIC), Aqueous Normal Phase (ANP) and Supercritical Fluid Chromatography (SFC). This course
allows you to explore the various options available to expand the polarity range of molecules that
can be retained and discusses how these complement standard reversed-phase methodology.
鈥� Limitations of reversed-phase HPLC 鈥� HILIC
鈥� Alternatives to C18 鈥� Aqueous normal-phase
鈥� Ion pairing 鈥� SFC
Available as an in-house or off-site course. Contact us for details
Tel: 0118 930 3660 Email: seminars@hichrom.co.uk
